MicroRNA regulation of endothelin-1 mRNA in renal collecting duct cells.

ABSTRACT:

Aims

Recently, microRNAs (miRNAs) have been implicated in control of Edn1 mRNA in several tissues. Here we examined the role of miRNA action on Edn1 mRNA expression in renal distal collecting duct cells.

Main methods

A microarray study was conducted to provide a comprehensive assessment of miRNAs present in a murine inner medullary collecting duct (mIMCD-3) cell line. The experiment was designed as a comparison between mIMCD-3 cells grown in the presence and absence of aldosterone. Argonaute (Ago) immunoprecipitation experiments were used to investigate binding of the RNA induced silencing complex (RISC) to Edn1 mRNA.

Key findings

Thirty-four miRNAs were detected in very high abundance in mIMCD-3 cells, and a large number of others were present at lower levels. The mi

SUBMITTER: Jacobs ME 

PROVIDER: S-EPMC4163143 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature