Vaccine delivery to the oral cavity using coated microneedles induces systemic and mucosal immunity.

ABSTRACT:

Purpose

The objective of this study is to evaluate the feasibility of using coated microneedles to deliver vaccines into the oral cavity to induce systemic and mucosal immune responses.

Method

Microneedles were coated with sulforhodamine, ovalbumin and two HIV antigens. Coated microneedles were inserted into the inner lower lip and dorsal surface of the tongue of rabbits. Histology was used to confirm microneedle insertion, and systemic and mucosal immune responses were characterized by measuring antigen-specific immunoglobulin G (IgG) in serum and immunoglobulin A (IgA) in saliva, respectively.

Results

Histological evaluation of tissues shows that coated microneedles can penetrate the lip and tongue to deliver coatings. Using ovalbumin as a model antigen it was found

SUBMITTER: Ma Y 

PROVIDER: S-EPMC4163144 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature