Functional screening of antibiotic resistance genes from a representative metagenomic library of food fermenting microbiota.

ABSTRACT: Lactic acid bacteria (LAB) represent the predominant microbiota in fermented foods. Foodborne LAB have received increasing attention as potential reservoir of antibiotic resistance (AR) determinants, which may be horizontally transferred to opportunistic pathogens. We have previously reported isolation of AR LAB from the raw ingredients of a fermented cheese, while AR genes could be detected in the final, marketed product only by PCR amplification, thus pointing at the need for more sensitive microbial isolation techniques. We turned therefore to construction of a metagenomic library containing microbial DNA extracted directly from the food matrix. To maximize yield and purity and to ensure that genomic complexity of the library was representative of the original bacterial population, we d

SUBMITTER: Devirgiliis C 

PROVIDER: S-EPMC4163480 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature