Developing conversed microsatellite markers and their implications in evolutionary analysis of the Bemisia tabaci complex.

ABSTRACT: The study of population genetics among the Bemisia tabaci complex is limited due to the lack of conserved molecular markers. In this study, 358, 433 and 322 new polynucleotide microsatellites are separately identified from the transcriptome sequences of three cryptic species of the B. tabaci complex. The cross species transferability of 57 microsatellites was then experimentally validated. The results indicate that these markers are conserved and have high inter-taxon transferability. Thirteen markers were employed to assess the genetic relationships among six cryptic species of the B. tabaci complex. To our surprise, the inferred phylogeny was consistent with that of mitochondrial COI sequences, indicating that microsatellites have the potential to distinguish species of the B. tabaci com

SUBMITTER: Wang HL 

PROVIDER: S-EPMC4163675 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature