Broad anti-HIV activity of the Oscillatoria agardhii agglutinin homologue lectin family.

ABSTRACT:

Objectives

Oscillatoria agardhii agglutinin homologue (OAAH) proteins belong to a recently discovered lectin family. The founding member OAA and a designed hybrid OAAH (OPA) recognize similar but unique carbohydrate structures of Man-9, compared with other antiviral carbohydrate-binding agents (CBAs). These two newly described CBAs were evaluated for their inactivating properties on HIV replication and transmission and for their potential as microbicides.

Methods

Various cellular assays were used to determine antiviral activity against wild-type and certain CBA-resistant HIV-1 strains: (i) free HIV virion infection in human T lymphoma cell lines and PBMCs; (ii) syncytium formation assay using persistently HIV-infected T cells and non-infected CD4+ T cells; (iii) DC-SIGN-medi

SUBMITTER: Ferir G 

PROVIDER: S-EPMC4164143 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature