Respiratory tract clinical sample selection for microbiota analysis in patients with pulmonary tuberculosis.

ABSTRACT:

Background

Changes in respiratory tract microbiota have been associated with diseases such as tuberculosis, a global public health problem that affects millions of people each year. This pilot study was carried out using sputum, oropharynx, and nasal respiratory tract samples collected from patients with pulmonary tuberculosis and healthy control individuals, in order to compare sample types and their usefulness in assessing changes in bacterial and fungal communities.

Findings

Most V1-V2 16S rRNA gene sequences belonged to the phyla Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Fusobacteria, with differences in relative abundances and in specific taxa associated with each sample type. Most fungal ITS1 sequences were classified as Ascomycota and Basidiomycot

SUBMITTER: Botero LE 

PROVIDER: S-EPMC4164332 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature