Overexpression of mouse angiotensinogen in renal proximal tubule causes salt-sensitive hypertension in mice.

ABSTRACT:

Background

The role of proximal tubule (PT) angiotensinogen (AGT) in modulating blood pressure has previously been examined using mice expressing PT human AGT and human renin, or rat AGT. These animals are hypertensive; however, the question remains whether alterations in mouse PT AGT alone affects arterial pressure.

Methods

Mouse AGT cDNA was knocked-in to the endogenous kidney androgen protein (KAP) gene using an internal ribosomal entry site (IRES)-based strategy.

Results

The KAP-mAGT animals showed kidney-specific KAP-AGT mRNA expression; renal in situ hybridization detected KAP-AGT mRNA only in PT. Urinary AGT was markedly increased in KAP-mAGT mice. On a high Na diet, radiotelemetric arterial pressure showed a systolic pressure elevation; no significant differen

SUBMITTER: Ying J 

PROVIDER: S-EPMC4164431 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature