Intra-operative preparation of autologous bone marrow-derived CD34-enriched cellular products for cardiac therapy.

ABSTRACT:

Background and aims

With the advent of regenerative therapy, there is renewed interest in the use of bone marrow as a source of adult stem and progenitor cells, including cell subsets prepared by immunomagnetic selection. Cell selection must be rapid, efficient and performed according to current good manufacturing practices. In this report we present a methodology for intra-operative preparation of CD34(+) selected autologous bone marrow for autologous use in patients receiving coronary artery bypass grafts or left ventricular assist devices.

Methods and results

We developed a rapid erythrocyte depletion method using hydroxyethyl starch and low-speed centrifugation to prepare large-scale (mean 359 mL) bone marrow aspirates for separation on a Baxter Isolex 300i immunomagneti

SUBMITTER: Donnenberg AD 

PROVIDER: S-EPMC4165076 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature