Selective mitogen activated protein kinase activity sensors through the application of directionally programmable D domain motifs.

ABSTRACT: Accurate and quantitative methods for measuring the dynamic fluctuations of protein kinase activities are critically needed as diagnostic tools and for the evaluation of kinase-targeted inhibitors, which represent a major therapeutic development area in the treatment of cancer and other diseases. In particular, rapid and economical methods that utilize simple instrumentation and provide quantitative data in a high throughput format will have the most impact on basic research in systems biology and medicine. There are over 500 protein kinases in the human kinome. Among these, the mitogen activated protein (MAP) kinases are recognized to be central players in key cellular signaling events and are associated with essential processes including growth, proliferation, differentiation, migration,

SUBMITTER: Peterson LB 

PROVIDER: S-EPMC4165445 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature