Evaluation of RT-PCR and immunohistochemistry as tools for detection of enterovirus in the human pancreas and islets of Langerhans.

ABSTRACT:

Background

Enteroviruses have been implicated in the etiology of type 1 diabetes, supported by immunoreactivity of enteroviral protein in islets, but presence of enteroviral genome has rarely been reported. Failure to detect enterovirus with RT-PCR has been attributed to the possible presence of PCR inhibitors and that only few cells are infected.

Objectives

The aim of this study was to evaluate strategies for detection of enterovirus in human islets.

Study design

A scenario was modeled with defined infected islets among a large number of uninfected pancreatic cells and the sensitivity of immunohistochemistry and PCR for detection of enterovirus was evaluated.

Results

Enterovirus was detected with PCR when only one single human islet, infected in vitro with a l

SUBMITTER: Skog O 

PROVIDER: S-EPMC4165708 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature