Transcriptomic dissection of the rice-Burkholderia glumae interaction.

ABSTRACT:

Background

Bacterial panicle blight caused by the bacterium Burkholderia glumae is an emerging disease of rice in the United States. Not much is known about this disease, the disease cycle or any source of disease resistance. To understand the interaction between rice and Burkholderia glumae, we used transcriptomics via next-generation sequencing (RNA-Seq) and bioinformatics to identify differentially expressed transcripts between resistant and susceptible interactions and formulate a model for rice resistance to the disease.

Results

Using inoculated young seedlings as sample tissues, we identified unique transcripts involved with resistance to bacterial panicle blight, including a PIF-like ORF1 and verified differential expression of some selected genes using qRT-PCR. These

SUBMITTER: Magbanua ZV 

PROVIDER: S-EPMC4165909 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature