Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection.

ABSTRACT: Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable technique for the routine identification of pathogenic yeasts. The main aim of this study was to apply MALDI-TOF MS combined with classical phenotypic and molecular approaches to identify Candida clinical isolates preserved from 1 up to 52 years in a Brazilian culture collection and assess its value for the identification of yeasts preserved in this type of collections. Forty Candida spp. clinical isolates were identified by morphologica

SUBMITTER: Lima-Neto R 

PROVIDER: S-EPMC4166277 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature