Gestational diabetes mellitus alters apoptotic and inflammatory gene expression of trophobasts from human term placenta.

ABSTRACT:

Aim

Increased placental growth secondary to reduced apoptosis may contribute to the development of macrosomia in GDM pregnancies. We hypothesize that reduced apoptosis in GDM placentas is caused by dysregulation of apoptosis related genes from death receptors or mitochondrial pathway or both to enhance placental growth in GDM pregnancies.

Methods

Newborn and placental weights from women with no pregnancy complications (controls; N=5), or with GDM (N=5) were recorded. Placental villi from both groups were either fixed for TUNEL assay, or snap frozen for gene expression analysis by apoptosis PCR microarrays and qPCR.

Results

Maternal, placental and newborn weights were significantly higher in the GDM group vs. Controls. Apoptotic index of placentas from the GDM group wa

SUBMITTER: Magee TR 

PROVIDER: S-EPMC4166519 | biostudies-literature | 2014 Jul-Aug

REPOSITORIES: biostudies-literature