A distal region of the human TGM1 promoter is required for expression in transgenic mice and cultured keratinocytes.

ABSTRACT:

Background

TGM1(transglutaminase 1) is an enzyme that crosslinks the cornified envelope of mature keratinocytes. Appropriate expression of the TGM1 gene is crucial for proper keratinocyte function as inactivating mutations lead to the debilitating skin disease, lamellar ichthyosis. TGM1 is also expressed in squamous metaplasia, a consequence in some epithelia of vitamin A deficiency or toxic insult that can lead to neoplasia. An understanding of the regulation of this gene in normal and abnormal differentiation states may contribute to better disease diagnosis and treatment.

Methods

In vivo requirements for expression of the TGM1 gene were studied by fusing various lengths of promoter DNA to a reporter and injecting the DNA into mouse embryos to generate transgenic animals.

SUBMITTER: Phillips MA 

PROVIDER: S-EPMC416661 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature