Interictal spikes precede ictal discharges in an organotypic hippocampal slice culture model of epileptogenesis.

ABSTRACT: In organotypic hippocampal slice cultures, principal neurons form aberrant excitatory connections with other principal cells in response to slicing induced deafferentation, similar to mechanisms underlying epileptogenesis in posttraumatic epilepsy. To investigate the consequences of this synaptogenesis, the authors recorded field-potential activity from area CA3 during perfusion with the complete growth medium used during incubation. At 7 days in vitro, slice cultures only displayed multiunit activity. At 14 days in vitro, the majority displayed population bursts reminiscent of interictal-like spikes, but sustained synchronous activity was rare. Band-pass filtering of interictal discharges revealed fast ripple-like complexes, similar to in vivo recordings. Spontaneous ictal-like activity b

SUBMITTER: Dyhrfjeld-Johnsen J 

PROVIDER: S-EPMC4167405 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature