Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli.

ABSTRACT: The ferric uptake regulator (Fur) plays a critical role in the transcriptional regulation of iron metabolism. However, the full regulatory potential of Fur remains undefined. Here we comprehensively reconstruct the Fur transcriptional regulatory network in Escherichia coli K-12 MG1655 in response to iron availability using genome-wide measurements. Integrative data analysis reveals that a total of 81 genes in 42 transcription units are directly regulated by three different modes of Fur regulation, including apo- and holo-Fur activation and holo-Fur repression. We show that Fur connects iron transport and utilization enzymes with negative-feedback loop pairs for iron homeostasis. In addition, direct involvement of Fur in the regulation of DNA synthesis, energy metabolism and biofilm develop

SUBMITTER: Seo SW 

PROVIDER: S-EPMC4167408 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature