Cell surface topography is a regulator of molecular interactions during chemokine-induced neutrophil spreading.

ABSTRACT: Adhesive interactions between neutrophils and endothelium involve chemokine-induced neutrophil spreading and subsequent crawling on the endothelium to sites of transmigration. We investigated the importance of cell topography in this process using immunofluorescence, scanning electron microscopy, and live-cell imaging using total internal reflectance microscopy to observe redistribution of key membrane proteins, both laterally and relative to surface topography, during neutrophil spreading onto glass coated with interleukin 8. During formation of the lamellipod, L-selectin is distributed on microvilli tips along the top of the lamellipodium, whereas the interleukin 8 receptors CXCR1 and CXCR2 and the integrin LFA-1 (αLβ2) were present at the interface between the lamellipodium and the subs

SUBMITTER: Lomakina EB 

PROVIDER: S-EPMC4167532 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature