Human airway musculature on a chip: an in vitro model of allergic asthmatic bronchoconstriction and bronchodilation.

ABSTRACT: Many potential new asthma therapies that show promise in the pre-clinical stage of drug development do not demonstrate efficacy during clinical trials. One factor contributing to this problem is the lack of human-relevant models of the airway that recapitulate the tissue-level structural and functional phenotypes of asthma. Hence, we sought to build a model of a human airway musculature on a chip that simulates healthy and asthmatic bronchoconstriction and bronchodilation in vitro by engineering anisotropic, laminar bronchial smooth muscle tissue on elastomeric thin films. In response to a cholinergic agonist, the muscle layer contracts and induces thin film bending, which serves as an in vitro analogue for bronchoconstriction. To mimic asthmatic inflammation, we exposed the engineered tis

SUBMITTER: Nesmith AP 

PROVIDER: S-EPMC4167568 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature