The structure of a neutralized virus: canine parvovirus complexed with neutralizing antibody fragment.

ABSTRACT:

Background

Members of the Parvovirus genus cause a variety of diseases in mammals, including humans. One of the major defences against viral infection is the presence of neutralizing antibodies that prevent virus particles from infecting target cells. The mechanism of neutralization is not well understood. We therefore studied the structure of canine parvovirus (CPV) complexed with the Fab fragment of a neutralizing antibody, A3B10, using image reconstruction of electron micrographs of vitrified samples, together with the already known structure of CPV from X-ray crystallographic data.

Results

The structure of the complex of CPV with Fab A3B10 has been determined to 23 A resolution. The known CPV atomic structure was subtracted from the electron density of the complex, and t

SUBMITTER: Wikoff WR 

PROVIDER: S-EPMC4167666 | biostudies-literature | 1994 Jul

REPOSITORIES: biostudies-literature