Chemical reporter for visualizing metabolic cross-talk between carbohydrate metabolism and protein modification.

ABSTRACT: Metabolic chemical reporters have been largely used to study posttranslational modifications. Generally, it was assumed that these reporters entered one biosynthetic pathway, resulting in labeling of one type of modification. However, because they are metabolized by cells before their addition onto proteins, metabolic chemical reporters potentially provide a unique opportunity to read-out on both modifications of interest and cellular metabolism. We report here the development of a metabolic chemical reporter 1-deoxy-N-pentynyl glucosamine (1-deoxy-GlcNAlk). This small-molecule cannot be incorporated into glycans; however, treatment of mammalian cells results in labeling of a variety proteins and enables their visualization and identification. Competition of this labeling with sodium aceta

SUBMITTER: Zaro BW 

PROVIDER: S-EPMC4168799 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature