A fully synthetic human Fab antibody library based on fixed VH/VL framework pairings with favorable biophysical properties.

ABSTRACT: This report describes the design, generation and testing of Ylanthia, a fully synthetic human Fab antibody library with 1.3E+11 clones. Ylanthia comprises 36 fixed immunoglobulin (Ig) variable heavy (VH)/variable light (VL) chain pairs, which cover a broad range of canonical complementarity-determining region (CDR) structures. The variable Ig heavy and Ig light (VH/VL) chain pairs were selected for biophysical characteristics favorable to manufacturing and development. The selection process included multiple parameters, e.g., assessment of protein expression yield, thermal stability and aggregation propensity in fragment antigen binding (Fab) and IgG1 formats, and relative Fab display rate on phage. The framework regions are fixed and the diversified CDRs were designed based on a systemati

SUBMITTER: Tiller T 

PROVIDER: S-EPMC4169037 | biostudies-literature | 2013 May-Jun

REPOSITORIES: biostudies-literature