Immunophenotyping of peripheral eosinophils demonstrates activation in eosinophilic esophagitis.

ABSTRACT:

Background and aim

Eosinophilic esophagitis (EoE) is a chronic inflammatory disorder characterized by upper gastrointestinal symptoms and the presence of high numbers of eosinophils in the esophagus. Although eosinophils in the esophagus have been found to be activated in subjects with EoE, detailed studies of intracellular signaling pathways involved in the mechanism of activation of eosinophils in EoE have heretofore been limited. The aim of the study was to assess whether any surface molecules or transcription factors are activated in peripheral eosinophils in subjects with EoE.

Methods

Eosinophils and CD3+ lymphocytes were identified directly from 50 μL of whole blood of EoE and control subjects. Using Hi-FACS, levels of surface activation markers, including CD66b, and i

SUBMITTER: Nguyen T 

PROVIDER: S-EPMC4169142 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature