Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications.

ABSTRACT: This article describes a method to quantify the movements of larval zebrafish in multiwell plates, using the open-source MATLAB applications LSRtrack and LSRanalyze. The protocol comprises four stages: generation of high-quality, flatly illuminated video recordings with exposure settings that facilitate object recognition; analysis of the resulting recordings using tools provided in LSRtrack to optimize tracking accuracy and motion detection; analysis of tracking data using LSRanalyze or custom MATLAB scripts; and implementation of validation controls. The method is reliable, automated and flexible, requires <1 h of hands-on work for completion once optimized and shows excellent signal:noise characteristics. The resulting data can be analyzed to determine the following: positional preferen

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC4169233 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature