The transcriptional profiling of glycogenes associated with hepatocellular carcinoma metastasis.

ABSTRACT:

Background and objective

Metastasis is one of the important reasons for the poor prognosis of hepatocellular carcinoma (HCC), abnormal glycosylation plays a pivotal role in HCC metastasis. The goal of this study was to screen and validate the transcriptional profiling of glycogenes associated with HCC metastasis.

Methodology

The differentially transcribed glycogenes were screened out by the Human Glycosylation RT2 Profiler PCR Array, and were identified by qRT-PCR in human HCC cell lines and their orthotopic xenograft tumors. Further analyses were performed with K-mean clustering, Gene Ontology (GO) and ingenuity pathways analysis (IPA). Four differentially transcribed glycogenes were validated in clinical cancer specimens by qRT-PCR.

Results

A total of thirty-three d

SUBMITTER: Liu T 

PROVIDER: S-EPMC4169445 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature