Comparative phenotypic analysis of the major fungal pathogens Candida parapsilosis and Candida albicans.

ABSTRACT: Candida parapsilosis and Candida albicans are human fungal pathogens that belong to the CTG clade in the Saccharomycotina. In contrast to C. albicans, relatively little is known about the virulence properties of C. parapsilosis, a pathogen particularly associated with infections of premature neonates. We describe here the construction of C. parapsilosis strains carrying double allele deletions of 100 transcription factors, protein kinases and species-specific genes. Two independent deletions were constructed for each target gene. Growth in >40 conditions was tested, including carbon source, temperature, and the presence of antifungal drugs. The phenotypes were compared to C. albicans strains with deletions of orthologous transcription factors. We found that many phenotypes are shared betwe

SUBMITTER: Holland LM 

PROVIDER: S-EPMC4169492 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature