Parallel single cancer cell whole genome amplification using button-valve assisted mixing in nanoliter chambers.

ABSTRACT: The heterogeneity of tumor cells and their alteration during the course of the disease urges the need for real time characterization of individual tumor cells to improve the assessment of treatment options. New generations of therapies are frequently associated with specific genetic alterations driving the need to determine the genetic makeup of tumor cells. Here, we present a microfluidic device for parallel single cell whole genome amplification (pscWGA) to obtain enough copies of a single cell genome to probe for the presence of treatment targets and the frequency of its occurrence among the tumor cells. Individual cells were first captured and loaded into eight parallel amplification units. Next, cells were lysed on a chip and their DNA amplified through successive introduction of dedi

SUBMITTER: Yang Y 

PROVIDER: S-EPMC4169497 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature