Multi-level kinetic model of mRNA delivery via transfection of lipoplexes.

ABSTRACT: Recent work on the use of mRNA lipoplexes for gene delivery demonstrates the need for a mathematical model that simulates and predicts kinetics and transfection efficiency. The small copy numbers involved make it necessary to use stochastic models and include statistical analysis of the variation observed in the experimental data. The modeling requirements are further complicated by the multi-level nature of the problem, where mRNA molecules are contained in lipoplexes, which are in turn contained in endosomes, where each of these entities displays a behavior of its own. We have created a mathematical model that reproduces both the time courses and the statistical variance observed in recent experiments using single-cell tracking of GFP expression after transfection. By applying a few key

SUBMITTER: Ligon TS 

PROVIDER: S-EPMC4169511 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature