A serum microRNA panel as potential biomarkers for hepatocellular carcinoma related with hepatitis B virus.

ABSTRACT:

Background

The identification of new high-sensitivity and high-specificity markers for HCC are essential. We aimed to identify serum microRNAs (miRNAs) as biomarkers to be used in diagnosing hepatitis B virus (HBV) -related hepatocellular carcinoma (HCC).

Methods

We investigated serum miRNA expression in (261 HCC patients, 233 cirrhosis patients, and 173 healthy controls), recruited between August 2010 and June 2013. An initial screening of miRNA expression by Illumina sequencing was performed using serum samples pooled from HCC patients and controls. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to evaluate the expression of selected miRNAs. A logistic regression model was constructed using a training cohort (n = 357) and then validated usi

SUBMITTER: Tan Y 

PROVIDER: S-EPMC4169601 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature