Small nucleolar RNA 113-1 suppresses tumorigenesis in hepatocellular carcinoma.

ABSTRACT:

Background

Emerging evidence suggests that small nucleolar RNAs (snoRNAs) are involved in tumorigenesis. The roles of small nucleolar RNA 113-1 (SNORD113-1) on the development of hepatocellular carcinoma (HCC) remain unknown.

Methods

The expression of SNORD113-1 was measured in 112 HCC tumor tissues using quantitative RT-PCR and compared with expression levels from with paired non-tumor tissues. The effects of SNORD113-1 on HCC tumorigenesis were investigated in HepG2 and Huh7 cells as well as a xenograft nude mouse model. CpG methylation within the promoter region of the SNORD113-1 gene was identified using Sodium bisulfite sequencing. Cancer pathway reporter investigate the mechanism by which SNORD113-1 suppressed tumorigenesis.

Results

SNORD113-1 expression was sig

SUBMITTER: Xu G 

PROVIDER: S-EPMC4169825 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature