Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina.

ABSTRACT:

Purpose

Simultaneous dual labeling to visualize specific RNA and protein content within the same formalin-fixed paraffin embedded (FFPE) section can be technically challenging and usually impossible, because of variables such as tissue fixation time and pretreatment methods to access the target RNA or protein. Within a specific experiment, ocular tissue sections can be a precious commodity. Thus, the ability to easily and consistently detect and localize cell-specific expression of RNA and protein within a single slide would be advantageous. In this study, we describe a simplified and reliable method for combined in situ hybridization (ISH) and immunohistochemistry (IHC) for detection of mRNA and protein, respectively, within the same FFPE ocular tissue.

Methods

Whole mouse

SUBMITTER: Stempel AJ 

PROVIDER: S-EPMC4169891 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature