Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies.

ABSTRACT: Turner syndrome is caused by complete or partial loss of the second sex chromosome and is characterized by spontaneous fetal loss in >90% of conceptions. Survivors possess an array of somatic and germline clinical characteristics. Induced pluripotent stem cells (iPSCs) offer an opportunity for insight into genetic requirements of the X chromosome linked to Turner syndrome. We derived iPSCs from Turner syndrome and control individuals and examined germ cell development as a function of X chromosome composition. We demonstrate that two X chromosomes are not necessary for reprogramming or maintenance of pluripotency and that there are minimal differences in gene expression, at the single cell level, linked to X chromosome aneuploidies. Formation of germ cells, as assessed in vivo through a mu

SUBMITTER: Dominguez AA 

PROVIDER: S-EPMC4170197 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature