Prognostic value of DNA alterations on chromosome 17p13.2 for intrahepatic cholangiocarcinoma.

ABSTRACT:

Aim

To characterize and evaluate DNA alterations among intrahepatic cholangiocarcinoma (ICC) patients.

Methods

DNA from tumor and corresponding normal tissues of 52 patients was amplified with 33 arbitrary primers. The DNA fragment that alters most frequently in ICC was cloned, sequenced, and identified by comparison with known nucleotide sequences in the genome database (www.ncbi.nlm.nih.gov). The DNA copy numbers of the allelic alterations in cholangiocarcinoma were determined by quantitative real-time PCR and interpreted as allelic loss or DNA amplification by comparison with the reference gene. Associations between allelic imbalance and clinicopathological parameters of ICC patients were evaluated by chi2-test. The Kaplan-Meier method was used to analyze survival rates.<

SUBMITTER: Chuensumran U 

PROVIDER: S-EPMC4171154 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature