Establishment and primary application of a mouse model with hepatitis B virus replication.

ABSTRACT:

Aim

To establish a rapid and convenient animal model with hepatitis B virus (HBV) replication.

Methods

A naked DNA solution of HBV-replication-competent plasmid was transferred to BALB/C mice via the tail vein, using a hydrodynamic in vivo transfection procedure. After injection, these mice were sacrificed on d 1, 3, 4, 5, 7 and 10. HBV DNA replication intermediates in the liver were analyzed by Southern blot hybridization. The expression of hepatitis B core antigen (HBcAg) and hepatitis B surface antigen (HBsAg) in the liver was checked by immunohistochemistry. Serum HBsAg and hepatitis B e antigen (HBeAg) was detected by enzyme-linked immunosorbent assay (ELISA). Inhibition of HBV replication was compared in HBV replication model mice treated intraperitoneally with polyino

SUBMITTER: Liu FJ 

PROVIDER: S-EPMC4171321 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature