The gene expression profile of cumulus cells reveals altered pathways in patients with endometriosis.

ABSTRACT:

Purpose

The objective of this experimental study was to compare the global gene expression profile of CC of mature oocytes in 18 patients with severe endometriosis and CC in 18 control patients affected by a severe male factor.

Methods

For each group, the CC were pooled, RNA was extracted and a microarray performed. For validating the microarray, a quantitative real-time PCR was performed in the CC of an independent set of patients with endometriosis (n = 5) and controls (n = 7).

Results

595 differentially expressed genes (320 down-regulated, 275 up-regulated, p < 0.05, fold change ≥1.5) were identified. The most significant changes were observed in genes involved in the chemokine signaling and cell-cell or cell-extracellular matrix adhesion pathways. Several genes of

SUBMITTER: Allegra A 

PROVIDER: S-EPMC4171411 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature