Determining the GmRIN4 requirements of the soybean disease resistance proteins Rpg1b and Rpg1r using a nicotiana glutinosa-based agroinfiltration system.

ABSTRACT: Rpg1b and Rpg1r are soybean disease resistance (R) genes responsible for conferring resistance to Pseudomonas syringae strains expressing the effectors AvrB and AvrRpm1, respectively. The study of these cloned genes would be greatly facilitated by the availability of a suitable transient expression system. The commonly used Niciotiana benthamiana-based system is not suitable for studying Rpg1b and Rpg1r function, however, because expression of AvrB or AvrRpm1 alone induces a hypersensitive response (HR), indicating that N. benthamiana contains endogenous R genes that recognize these effectors. To identify a suitable alternative host for transient expression assays, we screened 13 species of Nicotiana along with 11 accessions of N. tabacum for lack of response to transient expression of Avr

SUBMITTER: Kessens R 

PROVIDER: S-EPMC4171518 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature