Virus-mediated compartmentalization of the host translational machinery.

ABSTRACT:

Unlabelled

Viruses require the host translational apparatus to synthesize viral proteins. Host stress response mechanisms that suppress translation, therefore, represent a significant obstacle that viruses must overcome. Here, we report a strategy whereby the mammalian orthoreoviruses compartmentalize the translational machinery within virus-induced inclusions known as viral factories (VF). VF are the sites of reovirus replication and assembly but were thought not to contain ribosomes. It was assumed viral mRNAs exited the VF to undergo translation by the cellular machinery, and proteins reentered the factory to participate in assembly. Here, we used ribopuromycylation to visualize active translation in infected cells. These studies revealed that active translation occurs within VF

SUBMITTER: Desmet EA 

PROVIDER: S-EPMC4172071 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature