Antigen detection via the rate of ion current rectification change of the antibody-modified glass nanopore membrane.

ABSTRACT: Ion current rectification (ICR), defined as an increase in ion conduction at a given polarity and a decrease in ion conduction for the same voltage at the opposite polarity, i.e., a deviation from a linear ohmic response, occurs in conical shaped pores due to the voltage dependent solution conductivity within the aperture. The degree to which the ionic current rectifies is a function of the size and surface charge of the nanopore, with smaller and more highly charged pores exhibiting greater degrees of rectification. The ICR phenomenon has previously been exploited for biosensing applications, where the level of ICR for a nanopore functionalized with an analyte-specific binding molecule (e.g., an antibody, biotin, etc.) changes upon binding its target analyte (e.g., an antigen, streptavidi

SUBMITTER: Schibel AE 

PROVIDER: S-EPMC4172300 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature