Comprehensive analysis of preeclampsia-associated DNA methylation in the placenta.

ABSTRACT:

Background

A small number of recent reports have suggested that altered placental DNA methylation may be associated with early onset preeclampsia. It is important that further studies be undertaken to confirm and develop these findings. We therefore undertook a systematic analysis of DNA methylation patterns in placental tissue from 24 women with preeclampsia and 24 with uncomplicated pregnancy outcome.

Methods

We analyzed the DNA methylation status of approximately 27,000 CpG sites in placental tissues in a massively parallel fashion using an oligonucleotide microarray. Follow up analysis of DNA methylation at specific CpG loci was performed using the Epityper MassArray approach and high-throughput bisulfite sequencing.

Results

Preeclampsia-specific DNA methylation c

SUBMITTER: Chu T 

PROVIDER: S-EPMC4172433 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature