The statistical conformation of a highly flexible protein: small-angle X-ray scattering of S. aureus protein A.

ABSTRACT: Staphylococcal protein A (SpA) is a multidomain protein consisting of five globular IgG binding domains separated by a conserved six- to nine-residue flexible linker. We collected SAXS data on the N-terminal protein-binding half of SpA (SpA-N) and constructs consisting of one to five domain modules in order to determine statistical conformation of this important S. aureus virulence factor. We fit the SAXS data to a scattering function based on a new polymer physics model, which provides an analytical description of the SpA-N statistical conformation. We describe a protocol for systematically determining the appropriate level of modeling to fit a SAXS data set based on goodness of fit and whether the addition of parameters improves it. In the case of SpA-N, the analytical polymer physics de

SUBMITTER: Capp JA 

PROVIDER: S-EPMC4172662 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature