Difference in gene expression of macrophage between normal spleen and portal hypertensive spleen identified by cDNA microarray.

ABSTRACT:

Aim

To identify the difference in gene expression of microphage (Mphi) between normal spleen and portal hypertensive spleen using cDNA microarrays and find new gene functions associated with hypersplenism in portal hypertension.

Methods

The Biostar-H140s chip containing 14112 spots of cDNAs were used to investigate the difference of the expression. The total RNA extracted from macrophages isolated from both normal spleen and portal hypertensive spleen was reversely transcribed to cDNA with the incorporation of fluorescent (cy3 and cy5) labeled dCTP to prepare the hybridization probes. After hybridization, the gene chip was scanned for the fluorescent intensity. The differentially expressed genes were screened. That was repeated three times, and only the genes which had diffe

SUBMITTER: Yan F 

PROVIDER: S-EPMC4172719 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature