Cloning and tissue distribution of novel splice variants of the ovine ghrelin gene.

ABSTRACT: The ghrelin axis is involved in the regulation of metabolism, energy balance, and the immune, cardiovascular and reproductive systems. The manipulation of this axis has potential for improving economically valuable traits in production animals, and polymorphisms in the ghrelin (GHRL) and ghrelin receptor (GHSR) genes have been associated with growth and carcass traits. Here we investigate the structure and expression of the ghrelin gene (GHRL) in sheep, Ovis aries.We identify two ghrelin mRNA isoforms, which we have designated ?ex2 preproghrelin and ?ex2,3 preproghrelin. Expression of ?ex2,3 preproghrelin is likely to be restricted to ruminants, and would encode truncated ghrelin and a novel C-terminal peptide. Both ?ex2 preproghrelin and canonical preproghrelin mRNA isoforms were expressed in a range of tissues. Expression of the ?ex2,3 preproghrelin isoform, however, was restricted to white blood cells (WBC; where the wild-type preproghrelin isoform is not co-expressed), and gastrointestinal tissues. Expression of ?ex2 preproghrelin and ?ex2,3 preproghrelin mRNA was elevated in white blood cells in response to parasitic worm (helminth) infection in genetically susceptible sheep, but not in resistant sheep.The restricted expression of the novel preproghrelin variants and their distinct WBC expression pattern during parasite infection may indicate a novel link between the ghrelin axis and metabolic and immune function in ruminants.

SUBMITTER: Menzies M 

PROVIDER: S-EPMC4172912 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature