Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos.

ABSTRACT:

Background

Whole-mount in situ hybridization (WISH) is a fundamental tool for studying the spatio-temporal expression pattern of RNA molecules in intact embryos and tissues. The available methodologies for detecting mRNAs in embryos rely on enzymatic activities and chemical reactions that generate diffusible products, which are not fixed to the detected RNA, thereby reducing the spatial resolution of the technique. In addition, current WISH techniques are time-consuming and are usually not combined with methods reporting the expression of protein molecules.

Results

The protocol we have developed and present here is based on the RNAscope technology that is currently employed on formalin-fixed, paraffin-embedded and frozen tissue sections for research and clinical applications

SUBMITTER: Gross-Thebing T 

PROVIDER: S-EPMC4172952 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature