Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation.

ABSTRACT:

Background

Nasal secretion (NS) was investigated as a source of information regarding the mucosal and systemic immune status of cattle challenged by respiratory disease. A method for the collection of substantial volumes (~12 ml) of NS from cattle was developed to establish a reference range of analytes that are present in the NS of healthy cattle. Biochemical profiles of NS from a group of 38 healthy Holstein-Friesian cows revealed high alkaline phosphatase (AP) activity of up to 2392 IU/L. The character and source of the high activity of AP in bovine NS was investigated.

Results

Histochemical analysis confirmed the localization of the AP enzyme activity to epithelial cells and serous glands of the nasal respiratory mucosa. Analysis of mRNA levels from nasal mucosa by end p

SUBMITTER: Ghazali MF 

PROVIDER: S-EPMC4173004 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature