Preserving biological heterogeneity with a permuted surrogate variable analysis for genomics batch correction.

ABSTRACT:

Motivation

Sample source, procurement process and other technical variations introduce batch effects into genomics data. Algorithms to remove these artifacts enhance differences between known biological covariates, but also carry potential concern of removing intragroup biological heterogeneity and thus any personalized genomic signatures. As a result, accurate identification of novel subtypes from batch-corrected genomics data is challenging using standard algorithms designed to remove batch effects for class comparison analyses. Nor can batch effects be corrected reliably in future applications of genomics-based clinical tests, in which the biological groups are by definition unknown a priori.

Results

Therefore, we assess the extent to which various batch correction algori

SUBMITTER: Parker HS 

PROVIDER: S-EPMC4173013 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature