Large chromosomal rearrangements during a long-term evolution experiment with Escherichia coli.

ABSTRACT:

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Large-scale rearrangements may be important in evolution because they can alter chromosome organization and gene expression in ways not possible through point mutations. In a long-term evolution experiment, twelve Escherichia coli populations have been propagated in a glucose-limited environment for over 25 years. We used whole-genome mapping (optical mapping) combined with genome sequencing and PCR analysis to identify the large-scale chromosomal rearrangements in clones from each population after 40,000 generations. A total of 110 rearrangement events were detected, including 82 deletions, 19 inversions, and 9 duplications, with lineages having between 5 and 20 events. In three populations, successive rearrangements impacted particular regions. In five populations, rea

SUBMITTER: Raeside C 

PROVIDER: S-EPMC4173774 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature