Viral and host factors determine innate immune responses in airway epithelial cells from children with wheeze and atopy.

ABSTRACT:

Background

Airway epithelial cells (AEC) from patients with asthma, appear to have an impaired interferon (IFN)-β and -λ response to infection with rhinovirus.

Objectives

To determine if impaired IFN responses can be identified in young children at risk of developing asthma due to atopy and/or early life wheeze, and if the site of infection or the infecting virus influence the antiviral response.

Methods

Nasal (N) and tracheal (T) epithelial cells (EC) were collected from children categorised with atopy and/or wheeze based on specific IgE to locally common aeroallergens and a questionnaire concerning respiratory health. Submerged primary cultures were infected with respiratory syncytial virus (RSV) or human metapneumovirus (hMPV), and IFN production, inflammatory cyto

SUBMITTER: Spann KM 

PROVIDER: S-EPMC4174127 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature