The First Identified Citrus tristeza virus Isolate of Turkey Contains a Mixture of Mild and Severe Strains.

ABSTRACT: The presence of Citrus tristeza virus (CTV) has previously been reported in citrus growing regions of Turkey. All serologically and biologically characterized isolates including Iğdır, which was the first identified CTV isolates from Turkey, were considered mild isolates. In this study, molecular characteristics of the Iğdır isolate were determined by different methods. Analysis of the Iğdır isolate by western blot and BD-RT-PCR assays showed the presence of MCA13 epitope, predominantly found in severe isolates, in the Iğdır isolate revealing that it contains a severe component. For further characterization, the coat protein (CP) and the RNA-dependent RNA polymerase (RdRp) genes representing the 3' and 5' half of CTV genome, respectively, were amplified from dsRNA by RT-PCR. Both genes wer

SUBMITTER: Cevik B 

PROVIDER: S-EPMC4174788 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature