The DEK oncoprotein binds to highly and ubiquitously expressed genes with a dual role in their transcriptional regulation.

ABSTRACT:

Background

The DEK gene is highly expressed in a wide range of cancer cells, and a recurrent translocation partner in acute myeloid leukemia. While DEK has been identified as one of the most abundant proteins in human chromatin, its function and binding properties are not fully understood.

Methods

We performed ChIP-seq analysis in the myeloid cell line U937 and coupled it with epigenetic and gene expression analysis to explore the genome-wide binding pattern of DEK and its role in gene regulation.

Results

We show that DEK preferentially binds to open chromatin, with a low degree of DNA methylation and scarce in the heterochromatin marker H3K9me(3) but rich in the euchromatin marks H3K4me(2/3), H3K27ac and H3K9ac. More specifically, DEK binding is predominantly located

SUBMITTER: Sanden C 

PROVIDER: S-EPMC4175287 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature