Doxycycline enhances survival and self-renewal of human pluripotent stem cells.

ABSTRACT: We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance.

SUBMITTER: Chang MY 

PROVIDER: S-EPMC4175555 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature