6-Shogaol induces apoptosis in human leukemia cells through a process involving caspase-mediated cleavage of eIF2α.

ABSTRACT:

Background

6-Shogaol is a promising antitumor agent isolated from dietary ginger (Zingiber officinale). However, little is known about the efficacy of 6-shogaol on leukemia cells. Here we investigated the underlying mechanism of 6-shogaol induced apoptosis in human leukemia cells in vitro and in vivo.

Methods

Three leukemia cell lines and primary leukemia cells were used to investigate the apoptosis effect of 6-shogaol. A shotgun approach based on label-free proteome with LC-CHIP Q-TOF MS/MS was employed to identify the cellular targets of 6-shogaol and the differentially expressed proteins were analyzed by bioinformatics protocols.

Results

The present study indicated that 6-shogaol selectively induced apoptosis in transformed and primary leukemia cells but not in nor

SUBMITTER: Liu Q 

PROVIDER: S-EPMC4176122 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature